Abstract #1189

Fasciculus Axonal Connective Tissue Multiscale Imaging (FACTMI) - Connectome Mapping of Optic Nerve with 16 µm MRI at 14T and 0.1 µm histology

Walter Schneider¹, Yijen Wu¹, Alan Watson¹, Kasia Kedziora¹, Sudhir Pathak¹, Yongxin Zhao², Vijay Gorantla³, Jens Anders⁴, Rolf Polman⁵, and Klaus Scheffler⁵

¹University of Pittsburgh, Pittsburgh, PA, United States, ²Carnegie Mellon University, Pittsburgh, PA, United States, ³Wake Forest University, Winston-Salem, NC, United States, ⁴University of Stuttgart, Stuttgart, Germany, ⁵Max Planck Institute for Biological Cybernetics, Tübingen, Germany

Synopsis

Keywords: Quantitative Imaging, Brain Connectivity, Connectome, Histology, PhantomAccurate brain connectome mapping requires tracking fasciculus bundles of axons within tracts. In porcine optic nerve harvested tissue and TAXON diffusion phantom on a 14T magnet with a new linear coil array, we identify fasciculi with 16 µm resolution and follow TAXON fibers over centimeters from eye to LGN. MAGNIFY and bright field optical histology provide 0.1 & 0.25-micron resolution with accurate counts of the 1.2 million axons within fasciculi aligned with MRI and fasciculus wall structure. We use MRI and deep learning to predict the axon paths at each point and axon counts in each fasciculus.

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