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Abstract #1267

Whole Body Mouse EPR Oxygen Imaging of Implanted Beta Cell Replacement Devices

Mrignayani Kotecha1, Navin Viswakarma1, Safa Hameed1, Eliyas Siddiqui1, Feya Epel1, Cherie Stabler2, Minglin Ma3, and Boris Epel4
1Oxygen Measurement Core, O2M Technologies, LLC, Chicago, IL, United States, 2Department of Biomedical Engineering, University of Florida, Gainesville, IL, United States, 3Cornell University, Ithaca, NY, United States, 4Radiation and Cellular Oncology, The University of Chicago, Chicago, IL, United States

Synopsis

Keywords: Quantitative Imaging, Electron Paramagnetic Resonance, Oxygen Imaging, EPR imaging, Type I diabetes, cell encapsulation devicesBeta-cell replacement therapy remains the only approach with a clinical proof-of-concept that demonstrates long-term insulin independence can be achieved in type 1 diabetic (T1D) patients. The major challenge for beta cell replacement devices is to keep cells viable by avoiding hypoxia until vascularization is established. We hypothesize that by performing oxygen imaging and controlling the oxygenation of the devices early on, we can achieve better survival of beta cells and, consequently, better T1D reversal. EPROI is a quantitative oxygen imaging method that was used for obtaining pO2 maps in three different beta cell replacement devices in this study.

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