Keywords: Arterial spin labelling, Perfusion, test and retest reliability, multi-delay ASLThe goal of this study is to evaluate the quantification accuracy and measurement reliability of single- and multi-PLD pCASL via both simulation and in-vivo test and retest data. CBF calculated by single-delay ASL achieved highest test and retest reliability as compared to 5-PLD ASL. However, CBF values may be under-estimated due to prolonged ATT. 5-PLD ASL can be used to quantify ATT and CBF simultaneously with slightly reduced test and retest reliability. Model-fitting approach is more preferrable than the weighted-delay approach in terms of both quantification error and reliability.
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