Abstract #2988

The exchange rate of creatine CEST in mouse brain

Ziqin Zhang^1,2, Kexin Wang^1,2, Sooyeon Park^2,3, Anna Li², Yuguo Li^2,4, Robert Weiss^4,5, and Jiadi Xu^2,4

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States, ²F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Research Institute, Baltimore, MD, United States, ³Department of Neuroscience, Johns Hopkins University, Baltimore, MD, United States, ⁴Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁵Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States

Synopsis

Keywords: CEST & MT, CEST & MT, amide CEST, amine CEST, guanidinium CEST, creatine CEST, exchange rate, concentration, polynomial Lorentzian line-shape fitting (PLOF), high spectral resolution (HSR) CEST, two-step Bloch-McConnell (BM) fitting.We aim to use three different approaches to estimate the exchange rate of creatine (Cr) CEST, i.e., the pure CrCEST line-shape, B1-dependent CrCEST, and the pH response with different B1 values. The pure CrCEST signal extracted using wild type and GAMT-/- mice with low Cr and PCr concentrations; the pH in the brain cells altered by hypercapnia to demonstrate the pH sensitivity of GuanCEST; a two-step Bloch-McConnell fitting implemented to quantify the exchange rates. in vivo CrCEST exchange rate was found slow (~260-350 s-1). CrCEST is the major contribution to the opposite pH-dependence of GuanCEST signal under different B1.

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