Abstract #2993

The exchange rates of protein guanidinium protons in the mouse brain

Kexin Wang^1,2, Ran Sui^1,2, Lin Chen^3,4, Yuguo Li^2,3, and Jiadi Xu^2,3

¹Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States, ²F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Research Institute, Baltimore, MD, United States, ³Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, MD, United States, ⁴Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, China

Synopsis

Keywords: CEST & MT, Alzheimer's Disease, GuanCEST

We develop a novel two-step multi-B₁ Bloch-McConnell fitting approach for calculating the exchange rate of CEST protons in vivo, and apply it to guanidinium protons, the exchange rate of which is 70.1 ± 5.5 s^-1 with a concentration of 40.4 ± 5.2 mM in mouse brain at an optimized B₁ of 0.8 µT. Guanidinoacetate N-methyltransferase deficiency (GAMT^-/-) mice that have low creatine and phosphocreatine concentrations in brain are studied for protein guanidinium, i.e., arginineCEST (ArgCEST). The low exchange rate of ArgCEST suggests that the inverse pH dependence in GuanCEST with low B₁ is dominated by CrCEST compared to ArgCEST.

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