Abstract #3016

Hierarchical clustering analysis reveals size-dependent transport pathways of cerebrospinal fluid tracer in mouse brain

Yuran Zhu¹, Guanhua Wang², Yuning Gu¹, Huiyun Gao^3,4, Jing Zhang⁵, Yunmei Wang^3,4, Xiaofeng Zhu⁵, Chris A. Flask^1,6,7, and Xin Yu^1,6,8

¹Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States, ²Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, United States, ³Cardiovascular Research Institute, Case Western Reserve University, Cleveland, OH, United States, ⁴Department of Medicine, Case Western Reserve University, Cleveland, OH, United States, ⁵Department of Biostatistics, Population and Quantitative Health Sciences, Case Western Reserve University, Cleveland, OH, United States, ⁶Department of Radiology, Case Western Reserve University, Cleveland, OH, United States, ⁷Department of Pediatrics, Case Western Reserve University, Cleveland, OH, United States, ⁸Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH, United States

Synopsis

Keywords: Neurofluids, Contrast Agent, GlymphaticsThis study investigated the impact of molecular size on transport kinetics and distribution of intracisternal tracers in mouse brain. Three MRI contrast agents with different molecular sizes were administered via cisterna magna. Their transport in the whole brain was observed by dynamic contrast-enhanced MRI for 2 hours. Our results show that the transport of ¹⁷O-water (H₂¹⁷O) was significantly faster and more extensive than the two gadolinium-based tracers (Gd-DTPA and GadoSpin). Time-lagged correlation analysis and clustering analysis also showed different cluster patterns between Gd-DTPA and H₂¹⁷O. These observations suggest the size-dependent differences in forces that drive tracer transport in the brain.

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