Abstract #3174

Characterization and Optimization of New CEST Reporter Proteins with Increased Detection Specificity

David E. Korenchan¹, Adam Fillion², Leo L. Cheng³, Assaf A. Gilad², and Christian T. Farrar¹

¹Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, United States, ²Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI, United States, ³Department of Pathology, Massachusetts General Hospital, Charlestown, MA, United States

Synopsis

Keywords: CEST & MT, Cell Tracking & Reporter GenesCEST reporter genes could play a critical role in the optimization of novel biological therapeutics. However, existing amide proton based CEST reporter genes have poor specificity as the amide protons of the reporter gene have the same chemical shift as amide protons from endogenous proteins. Here we report on a new class of reporter proteins with CEST contrast at 5-ppm, based on tyrosine hydroxyl ring protons.

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