Keywords: Neuroinflammation, Brain, Diffusion Imaging, freewater, reliabilityMedical imaging can offer several clinically valuable biomarkers which require accuracy across the acquiring technologies. Our study investigated the variability and reliability of diffusion microstructures over different diffusion gradient direction schemes, including high angular resolution diffusion imaging sequences and a parallel slice acquisition technique. The study's results demonstrated the 20-directional parallel slice acquisition clinically feasible sequence for standard DTI analysis with less variability and more reliability in matrices, while multishell sequences are more suitable for microstructural and tractography analysis.
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