Keywords: Spectroscopy, Spectroscopy, ReproducibilityMulti-metabolite spectral-edited MRS enables the efficient detection of multiple low-concentration metabolites. Two such approaches, HERMES and HERCULES, directly edit two or more metabolites in a single scan. However, the reproducibility of these techniques has yet to be fully established. This study investigated PRESS- and sLASER-localized HERMES and HERCULES test-retest reliability, with the additional aim of demonstrating that sLASER localization is the better of the two sequences for obtaining reproducible measurements. Overall, the test-retest reliability of sLASER was found to be higher than that of PRESS.
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