Abstract #3742

A Novel MRI Platform for Long-term Stem Cell Tracking In Vivo

Keyu Zhuang^1,2, Rocco Romagnuolo³, Daniel A. Szulc^1,2, Hai-Ying Mary Cheng^4,5, Michael A. Laflamme^3,6,7, and Hai-Ling Margaret Cheng^1,2,8

¹Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada, ²Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada, ³McEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada, ⁴Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada, ⁵Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada, ⁶Peter Munk Cardiac Centre, University Health Network, Toronto, ON, Canada, ⁷Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, ⁸The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, Canada

Synopsis

Keywords: Molecular Imaging, Cell Tracking & Reporter Genes, stem cell

To extend our previously reported bright-ferritin cell tracking platform to monitoring stem cell therapy, we investigated tracking human embryonic stem cells transplanted intramuscularly in immune-compromised mice. With this technology, the stem cells impart a bright, T1-induced contrast. In-vitro assays of viability and pluripotency, and imaging both in vitro on cell pellets and in vivo in mice were performed. Monitoring mice on MRI over two months confirmed the ability to assess cell survival and teratoma formation non-invasively.

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