Keywords: Liver, Animals, assess fish healthQuantitative methods of fish lipid accumulation have attracted a lot of attention in ecotoxicology. Traditionally, invasive approaches were time-consuming and generally required animal sacrifice, drastically restricting applicability in ecotoxicology. In this study, a noninvasive MRI approach was proposed to quantify lipid accumulation in two represented fish species with IDEAL-IQ and Cube-Flex-T2 sequences. We demonstrated the accuracy of our novel MRI approach in quantifying fish lipid accumulation. In addition, we conducted cadmium exposure experiment and observed no significant differences in lipid deposition between MRI and traditional methods. Thus, this MRI method could have a wide range of applications in ecotoxicological research.
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