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Abstract #4022

Whole-brain fMRI study of mice under medetomidine/ketamine anesthesia to identify brain regions activated by musk odors

Sosuke Yoshinaga1, Yumiko Tsubakihara1, Takayuki Fukumoto1, Shotaro Maita1, Mitsuhiro Takeda1, and Hiroaki Terasawa1
1Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan

Synopsis

Keywords: New Devices, PreclinicalMuscone, a major component of musk odor, attracts male mice. The odor-evoked behaviors are mediated by neural circuits activated by the stimulation. We have been working on whole-brain BOLD fMRI studies of mice, using a method that employs periodic odor stimulation and independent component analysis. In this study, we investigated the brain regions activated by muscone in mice under medetomidine and low-dose ketamine anesthesia. Ketamine reportedly exhibits disinhibition of glutamate signaling at a low dose. As the result, we identified numerous muscone-evoked activated regions located in the olfactory pathways and higher-order regions in the cerebrum.

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Keywords