Keywords: Diffusion/other diffusion imaging techniques, Ex-Vivo Applications, fixation, PFA, in-situ MRI, whole human brainWe investigated the longitudinal fixation effect of 4% paraformaldehyde and phosphate-buffered saline on diffusion imaging-derived mean diffusivity (MD) and fractional anisotropy (FA) across deep gray, cortical gray and white matter in human brains using suboptimal and optimal diffusion protocols with varying maximal b-values.
We had two main findings: (1) a suboptimal diffusion protocol led to increased bias in the FA and MD up to 36.26% and 38.11%, the same trend was also observed for the across-brain variation; (2) MD changed by a maximum of 26.42% within the first 14 days in fixative and saturated afterward, whereas FA remained almost constant.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords