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Abstract #4263

A Strategy of Whole Blood Metabolomics Based on Magnetic Resonance Spectroscopy by Mixing Blood with Silica Powder

Leo Ling Cheng1,2, Xiaoyu Wang3,4, Zehan Liu3,4, Jessica Duffy4, Abner Louissaint, Jr.2,4, and Panteleimon Takis5
1Radiology, Massachusetts General Hospital, Boston, MA, United States, 2Harvard Medical School, Boston, MA, United States, 3Nanjing University, Nanjing, China, 4Molecular Pathology, Massachusetts General Hospital, Boston, MA, United States, 5Imperial College London, London, United Kingdom

Synopsis

Keywords: Screening, Spectroscopy, Gelified BiofluidsCurrently, blood metabolomics analysis has to choose between serum and plasma: the former leaves blood in room temperature and therefore causing metabolic alterations; and the latter has interfering chemicals added during acquisition of samples, which make results complicated. Here, using HRMAS MRS, we studied gel samples of whole mouse blood by mixing them with silica powder. The obtained spectra agreed, in general, with the correlating serum samples, which proved the robustness of this method. Taking advantage of tiny amounts of samples (μl) and simple and rapid pretreatment, our new technique demonstrates a novel approach to metabolomic analysis and clinical applications.

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