Keywords: Cartilage, Quantitative ImagingEx vivo and cadaveric articular cartilage samples often undergo freeze and thaw cycles and sample preparation in air. These procedures will result in cartilage dehydration and degradation, which may affect the results of UTE-biomarkers. This study was aimed to evaluate the effect of cartilage dehydration and rehydration on UTE-biomarkers and to compare the rehydration capability of synovial fluid and normal saline. Cartilage dehydration resulted in significant changes in all evaluated UTE-biomarkers. Rehydrating with normal saline had non-significant effect on UTE-biomarkers while synovial fluid resulted in significant changes for MMF and T2*. Rehydrating with normal saline is better than synovial fluid.
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