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Abstract #4550

Benchtop NMR for lab-on-a-chip

Marc Azagra1, Alejandro Portela1, Hetal Patel2, Dian Weerakonda2, Jose Yeste1, Alba Herrero-Gómez1, Matthew Fallon2, Megdouda Benamara3, Marc Dubois3, Tryfon Antonakakis3, Javier Ramon1, and Irene Marco-Rius1
1Institute for Bioengineering of Catalonia, Barcelona, Spain, 2Oxford Instruments, Abingdon, United Kingdom, 3Multiwave Imaging, Marseille, France

Synopsis

Keywords: New Devices, Hyperpolarized MR (Non-Gas), Molecular Imaging

A benchtop NMR spectrometer has been designed and optimized to monitor real-time metabolism of 3D tissue engineered on microfluidic platforms using hyperpolarization by dynamic nuclear polarisation. We show the design process, which included the modification of a commercial benchtop NMR spectrometer, the design and fabrication of a microfluidic platform for a reliable injection of a hyperpolarized substrate and a constant delivery and renewal of the cell media and its integration with a RF coil for transmit/receive, and the design and fabrication of a sample carrier. We also present our preliminary NMR data using this system.

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Keywords