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Abstract #4873

Ultra-high Moment Gold-coated Iron Particles Allows for Longitudinal Time-lapse MRI of Individual Stem Cells in the Brain

Nikorn Pothayee1, Stephen Dodd1, Li Liu1, Gary Zabow2, and Alan Koretsky1
1National Institutes of Health, Bethesda, MD, United States, 2National Institute of Standards and Technology, Boulder, CO, United States

Synopsis

Keywords: Contrast Agent, Cell Tracking & Reporter GenesIn this study, gold coated microfabricated gold-coated iron particles are used for in vitro and in vivo labeling of immune cell and neural precursors, respectively for MRI. These particles have a pure iron core and protective layer of gold and exhibit extremely high magnetic moment relative to more commonly used, chemically synthesized, iron-oxide based particles. Following in situ labeling of neural precursor cells, we show that the migration of a large pool of individual cells can be visualized in time-lapse and longitudinal MRI.

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Keywords