Abstract #5002

Minimally invasive measurement of arterial and brain temperature in the mouse

Lisa M Gazdzinski¹, Luke Chung^1,2, Shoshana Spring¹, Owen Botelho¹, Bojana Stefanovic^2,3, Brian J Nieman^1,2,4, Chinthaka C Heyn^3,5, and John G Sled^1,2

¹Mouse Imaging Centre, Hospital for Sick Children, Toronto, ON, Canada, ²Medical Biophysics, University of Toronto, Toronto, ON, Canada, ³Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, ⁴Ontario Institute for Cancer Research, Toronto, ON, Canada, ⁵Medical Imaging, University of Toronto, Toronto, ON, Canada

Synopsis

Keywords: Thermometry, Metabolism, lanthanide, mice, brainBrain temperature is an important physiological parameter that both reflects and modulates brain activity, but current in vivo thermometry methods are invasive and/or impractical for small animals. We present a minimally invasive method for measuring blood and brain temperature in mice using Tm-DOTMA and characterize the relationship between brain and blood temperature under isoflurane anesthesia. Brain temperature was strongly correlated and approximately equal to the temperature of the inflowing arterial blood, possibly reflecting the vasodilatory and metabolic suppressive effects of isoflurane. Application of this method with alternative anesthesia approaches would provide insight into brain temperature regulation in health and disease.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.