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Abstract #5013

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Mario Corral-Bolaños1,2, Tram Nguyen1, Hans Martin Kjer1, Marco Pizzolato1,2, Casper Bo Gravesen Salinas3, Johanna Perens3, Jeppe Revall Frisvad1, Julien Colombelli4, and Tim Bjørn Dyrby1,2
1Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kongens Lyngby, Denmark, 2Danish Research Centre for Magnetic Resonance (DRCMR), Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital, Amager & Hvidovre, Copenhagen, Denmark, 3Gubra, Hørsholm, Denmark, 4Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute for Science and Technology (BIST), Barcelona, Spain

Synopsis

Keywords: Validation, Tractography & Fibre Modelling, 3D-Fibre orientationThis work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

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