Keywords: Diffusion/other diffusion imaging techniques, Diffusion/other diffusion imaging techniques, G-ratioIn vivo MR g-ratio mapping relies on a calibrated method that relates myelin and axon MR markers to volume fractions. To estimate the calibration parameter, fixed ex vivo tissue measured with MRI and histology is used. The so-determined calibration parameter is then applied in vivo, neglecting the difference in the MR markers due to fixation. Here, we proposed a new calibration method accounting for this difference. The new calibration reduced the variability across MR g-ratios based on different myelin and axon markers, indicating that it can improve the accuracy of MR g-ratios.
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