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Abstract #5387

Absolute quantification of hepatic 31P metabolites at 3 T using a phantom replacement technique – How to set it up

Benedict Korzekwa1,2, Marc Jonuscheit1,2, Yuliya Kupriyanova1,2, Michael Roden1,2,3, and Vera B. Schrauwen-Hinderling1,2,4
1Institute for Clinical Diabetology, German Diabetes Center, Leibniz Institute for Diabetes Research at Heinrich Heine University, Düsseldorf, Germany, 2German Center for Diabetes Research (DZD e.V.), München-Neuherberg, Germany, 3Department of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany, 4Department of Radiology and Nuclear Medicine, Maastricht University Medical Center, Maastricht, Netherlands

Synopsis

Keywords: Data Analysis, Spectroscopy, 31P-MRS, Absolute Quantification; Quadrature Coil

Phantom replacement techniques for the quantification of absolute concentrations of hepatic phosphorous metabolites are an established tool for studying energy metabolism in vivo. However, many consideration need to be taken into account. Here, we present how to correct localized in vivo 31P-MRS data in order to report molar concentrations of ATP and inorganic phosphate and how to set up the experiments for obtaining the required correction factors. Correction factors include T1 correction, coil loading, excitation pulse profile as well as B1 inhomogeneity of the surface coil in all three spatial directions.

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Keywords