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Abstract #0212

Astrocytes contribute to signals of Hyperpolarized 13C pyruvate in the brain

Maiko Ono1, Bolati Wulaer2, Tomoteru Yamasaki3, Toshihiro Sakamoto1, Rikita Araki4, Kosei Hirata5, Keita Saito1, Yoichi Takakusagi1, Ming-Ron Zhan3, Jun Nagai2, and Yuhei Takado1
1Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, Chiba, Japan, 2RIKEN Center for Brain Science, Wako, Japan, 3Department of Advanced Nuclear Medicine Sciences, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology, Chiba, Japan, 4Bruker Japan K.K., Yokohama, Japan, 5National Institutes for Quantum Science and Technology, Chiba, Japan

Synopsis

Keywords: Hyperpolarized MR (Non-Gas), Hyperpolarized MR (Non-Gas)

Motivation: Hyperpolarized MR (HP-MR) 13C pyruvate is a valuable probe for evaluating glycolytic flux, but it remains unknown which cells contribute to the HP-MR signals in the brain.

Goal(s): To verify whether astrocyte metabolism is involved in the signal of HP-MR 13C pyruvate in the brain.

Approach: We perturbed Gq-GPCR/calcium (Ca2+) signaling in astrocytes using the hM3Dq DREADD system and examined the fluctuations of the HP-MR pyruvate signals in awake mice.

Results: In HP-MR experiments conducted 30 minutes after the elevation of astrocyte Ca2+, the lactate/pyruvate ratio decreased compared to the control, and the bicarbonate/pyruvate ratio also decreased.

Impact: Investigating the involvement of astrocyte metabolism in brain hyperpolarized MR(HP-MR) 13C pyruvate signals, astrocyte GPCR/Ca2+ signaling was perturbed using DREADD, revealing a decrease in lactate/pyruvate and bicarbonate/pyruvate ratios in HP-MR experiments, suggesting metabolic alterations in response to astrocyte GPCR/Ca2+ modulation.

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Abstract

Keywords