Abstract #1043

Validating DCE-MRI estimates of leakage volumes associated with subtle blood-brain barrier dysfunction using two-photon microscopy.

Martin Kozár^1,2, Sarah Al-Bachari³, Laura Parkes^2,4, Hervé Boutin^5,6, Ingo Schiessl^2,6, and Ben R Dickie^1,2

¹Division of Informatics, Imaging, and Data Sciences, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom, ²Geoffrey Jefferson Brain Research Center, Manchester Academic Health Science Center, The University of Manchester, Manchester, United Kingdom, ³University College London, London, United Kingdom, ⁴Division of Psychology, Communication and Human Neuroscience, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom, ⁵UMR 1253, iBrain, Inserm, Bat Planiol, UFR de Médecine, Université de Tours, Tours, France, ⁶Division of Neuroscience, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, United Kingdom

Synopsis

Keywords: Contrast Agents, DSC & DCE Perfusion

Motivation: DCE-MRI can be used to quantify subtle blood-brain barrier disruption. In this setting, it is generally assumed that contrast agent has access to the entire interstitial space. Prior work from our group indicates that contrast agent has access to a leakage volume much smaller than the interstitial space.

Goal(s): To provide independent validation of DCE-MRI leakage-to-vessel volume ratios (v_e/v_b) in the case of subtle BBB impairment.

Approach: The v_e/v_b ratio of Sulphorhodamine-101 (MW = 606.7g/mol) was measured in mouse brain using two-photon microscopy and compared to DCE-MRI estimates.

Results: The v_e/v_b ratio of sulphorhodamine-101 agrees well with DCE-MRI estimates.

Impact: Our results indicate that DCE-MRI kinetic models that assume infinite leakage volume (e.g. Patlak model) do not accurately reflect how Gd-DOTA distributes within the brain when BBB impairment is subtle.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.