Keywords: New Devices, Relaxometry
Motivation: Bioreactor cell density measurements currently require removing samples from the culture. This limits how many samples can be taken in a day and precludes real-time culture monitoring.
Goal(s): We sought to take nondestructive cell density measurements every ten minutes without removing cells from the bioreactor.
Approach: We built a system that takes T2 relaxometry measurements through the bioreactor’s silicone tubing every ten minutes and compared these to measurements taken by a commercial cytometry system.
Results: The T2 relaxation data closely tracks the true cell density. Changes made to the culture are detected much more quickly by the relaxometer.
Impact: Magnetic resonance relaxometry can be used to track bioreactor culture growth in real time, and makes it possible to identify and correct problems with the culture before the culture fails.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords