Keywords: Neurotransmission, Deuterium, Neurometabolism, brain, Neuroscience, Spectroscopy
Motivation: Lower sensitivity of 13C NMR spectroscopy requires longer acquisition time while quantifying brain metabolites. An alternative technique is essential to aid faster detection.
Goal(s): To evaluate the kinetics of 2H labeling brain metabolites from [6,6’-2H2]glucose using 2H NMR spectroscopy.
Approach: The cortical extracts of rats infused with [6,6’-2H2]glucose were analyzed using 2H NMR spectroscopy.
Results: The signals of deuterated GlcC6, GluC4, LacC3, GABAC2and GlnC4 are seen in the 2H NMR spectrum of cortical extract obtained after 90 min of [6,6’-2H2]glucose infusion. The resonances of AspC2, GluC2, and GluC3 are absent suggesting complete loss/dilution of 2H from the TCA cycle intermediates beyond α-ketoglutarate.
Impact: Judicious use of 2H NMR together with suitable deuterated substrates may an alternative for neurometabolic analysis in neurological disorders.
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