Keywords: Radiomics, Metabolism, 13C NMR, 13C isotopomer analysis
Motivation: The TCGA database reveals that gliomas exhibit the presence of phosphoglycerate dehydrogenase (PHGDH), a crucial enzyme in the generation of serine through de novo biosynthesis.
Goal(s): Our objective was to measure the serine biosynthesis flux in glioma patients.
Approach: We used 13C-glucose intravenous infusion during the surgical resection and 13C isotopomer analysis to track serine biosynthesis in patients.
Results: We detected 19.2% ± 6.5% of serine biosynthetic flux relative to lactate production and the generation of glycine to produce one-carbon units in both low-grade gliomas and GBMs.
Impact: These results indicate that targeting serine de novo synthesis may be of therapeutic value. We are currently working on developing PHGDH knockdown cell line from a GBM patient to test the effects of serine synthesis on cellular viability.
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