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Abstract #3214

Detection of low calcium channel activity in pig hearts with manganese-enhanced MRI

Wibeke Nordhøy1, Magne Mørk Kleppestø2, Tryggve Holck Storås2, and Jonny Østensen3
1Department of Physics and Computational Radiology, Oslo University Hospital, OSLO, Norway, 2Department of Physics and Computational Radiology, Oslo University Hospital, Oslo, Norway, 3IC Targets AS, Oslo, Norway

Synopsis

Keywords: Contrast Agents, Myocardium, MEMRI, Manganese, Mangafodipir, pig study, T tubuli, Ca2+ channel activity

Motivation: Evaluate manganese-enhanced MRI (MEMRI) for heart failure assessment.

Goal(s): Quantify the manganese uptake rate in the myocardium as an indicator of L-type calcium channel activity and T-tubule density.

Approach: A high temporally-resolved dynamic SatRec sequence was used to track the Mangafodipir bolus instead of using the less precise, but higher spatially-resolved, conventional T1 mapping method that was used before and after the manganese administration.

Results: Pigs have less dense T-tubule than humans leading to fewer L-type calcium channels and low Mn uptake in cardiomyocytes. Therefore, we believe that MEMRI can become a promising tool for assessing heart failure diseases in humans.

Impact: Since pigs are often used in preclinical research, there are important differences to be aware of. The low Mn uptake proved their less dense T-tubule, showing that MEMRI may be a useful tool for studying heart failure in humans.

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