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Abstract #3968

In-vivo Imaging of the adult Zebrafish in an unmodified pre-clinical MRI System at 11.7T

Julian Schüle1, Bernd Gahr2, Steffen Just2, Volker Rasche1, and Tobias Speidel1
1Internal Medicine 2, Ulm University Medical Center, Ulm, Germany, 2Molecular Cardiology, Ulm University Medical Center, Ulm, Germany

Synopsis

Keywords: Biology, Models, Methods, High-Field MRI

Motivation: The zebrafish is an important model organism for the study of vertebrate biology with its genomes showing significant parallels to human genomes.

Goal(s): To show the feasibility of in-vivo imaging at a pre-clinical 11.7T MRI without the need for dedicated hardware components.

Approach: A budget-friendly zebrafish holder was created using a syringe barrel and needle adapters, with a rotating cylinder to maintain water flow. It was positioned on a Bruker Mouse Brain Surface Coil.

Results: The use of standard imaging equipment in combination with a low-cost and easy to built animal holder is sufficient to facility high-resolution imaging of the adult zebrafish.

Impact: This research enables cost-effective, high-resolution MRI for zebrafish, enhancing its potential as a pre-clinical model for vital research in vertebrate biology, ultimately advancing our understanding of human genomics.

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