Meeting Banner
Abstract #3970

MALDI-MRS-SHY: Statistical Heterospectroscopy of MALDI Imaging and Magnetic Resonance Spectroscopy

Dalton Brown1, Zuriel Erikson Joven1, Natalie Dillman1, Ethan Yang1, Cole Johnson1, Sofia Nakuchima1, Caitlin Tressler1, and Kristine Glunde1
1Radiology, Johns Hopkins School of Medicine, Baltimore, MD, United States

Synopsis

Keywords: Biology, Models, Methods, Spectroscopy, MALDI imaging, heterospectroscopy

Motivation: Combining high-resolution (HR) magnetic resonance spectroscopy (MRS) and matrix-assisted laser desorption/ionization (MALDI) imaging of tissues and cells using new workflows and data analysis approaches will further advance metabolomic and lipidomic studies.

Goal(s): The goal is to develop Statistical Heterospectroscopy (SHY) of 1H-HR-MRS and MALDI imaging data of tissues and cells.

Approach: Phantoms, cell lines, and tumors were measured by 1H-HR-MRS and MALDI imaging, followed by analysis with newly developed MATLAB-based software for statistical correlation of both datasets.

Results: The new MALDI-MRS-SHY software correctly generated two-dimensional spectra with cross-peaks from phantoms of pure compounds and mixtures, as well as cell lines and tumor tissues.

Impact: The newly developed MALDI-MRS-SHY software will allow for a deeper analysis of complex metabolomic and lipidomic data obtained by combined HR-MRS and MALDI imaging of cells and tissues.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.

Keywords

negativecorrelationplotsoftwarecellsmetaboliteslaserpositivepurespectroscopytumordeveloped