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Abstract #3974

Time-dependent response of Silver nanoparticles reinforced biocomposite in Murine fibroblast cells by NMR-based Pharmacometabolomics

Isha Gupta1,2, Sonia Gandhi1, Abhishek Kumar1, Vijayakumar Chinnadurai1, Anant Narayan Bhatt1, and Sameer Sapra2
1Metabolomics Research Facility, Institute of Nuclear Medicine and Allied Sciences (INMAS), DRDO, Delhi, India, 2Department of Chemistry, Indian Institute of Technology Delhi (IITD), Delhi, India

Synopsis

Keywords: Other Preclinical, Toxicity, In vitro Pharmacometabolomics, NMR spectroscopy, Nanocomposite, Nanotoxicology

Motivation: Conventional toxicity assays generally convey the efficiency of nanomaterial, but they lack a mechanistic molecular approach to toxicity.

Goal(s): We aimed to employ NMR-based Pharmacometabolomics to detect early metabolic alterations associated with nanomaterial developed for healthcare.

Approach: The NMR spectroscopy technique was used on Silver nanoparticles reinforced biocomposite exposed murine fibroblast (L929) cells.

Results: Results demonstrated that cellular metabolic profiles reset towards control upon more exposure.

Impact: Our nanomaterial-based study accentuates the role of pharmacometabolomics, which will complement the findings of conventional assays, eventually leading to efficacious clinical translation.

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