1Laboratory of Functional and Metabolic
Imaging (LIFMET), Ecole Polytechnique Fdrale de Lausanne,
The aim of present study was to measure the macromolecule baselines from four different volumes of interest including cortex, hippocampus, striatum and a mixture of brain structures, and then to assess their influence on the quantification of metabolites. Minor differences were found between the macromolecules acquired from specific regions and a large volume containing various cerebral structures. A slight variability in the shape of the macromolecule baseline introduced by data processing can affect calculated concentrations of less well characterized metabolites. The use of a generic experimental macromolecule baseline provides a sufficiently accurate measurement of the neurochemical profile in rat brain.