Philippe Garteiser1, Bruce A. Berkowitz2,3, Debbie Saunders1, Rebecca Cranford1, Rheal A. Towner1, Michael H. Elliott4
1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2Department of Anatomy and Cell Biology, Wayne State University, Detroit, MI, United States; 3Department of Ophthalmology, Wayne State University, Detroit, MI, United States; 4Department of Ophtalmology, Dean A McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States
Mn-enhanced MRI (MEMRI) has recently emerged as an important tool in retinal function studies. Caveolin-1 (Cav-1), the principal protein member of caveolar membrane domains, is believed to be essential to blood-retinal barrier integrity and ion homeostasis of the retina. Here, we evaluate how MEMRI and other MRI techniques may detect functional disruptions induced by cell type-specific knock out of the Cav-1 gene in mice. The MEMRI signature of light and dark adaptation and the dynamic gadolinium-enhanced signal behavior of iodate-induced retinal impairments indicate that both methods have sufficient sensitivity to warrant their application to cell-type specific Cav-1 ko mice.