Eric Raymond Muir1,2, Bryan H. De La Garza2,
Sung-Hong Park3, Timothy Q. Duong2
1Biomedical Engineering, Georgia
Institute of Technology, Atlanta, GA, United States; 2Research
Imaging Institute, Ophthalmology/Radiology, UT Health Science Center San
Antonio, San Antonio, TX, United States; 3Research Imaging
Institute, Radiology, UT Health Science Center San Antonio, San Antonio, TX,
United States
Anatomical
MRI of the retina has previously reported 3-4 layers in the rodent retina
using conventional gradient echo (GE) and spin echo MRI. Following
intraocular injection of manganese, seven layers were detected previously.
This study explored balanced steady state free precession (bSSFP) MRI to
image the mouse retina at 35x35x200 m. Moreover, we compared GE and bSSFP
with and without intraocular manganese injection. We demonstrated that bSSFP
can reveal 7 layers without using contrast agent. Layers detected by bSSFP
without manganese were consistent with those of manganese-enhanced MRI and
histology.