Christopher M. Traudt1, Kathleen M. Ennis1, Raghu Rao1, Ivan Tkac2
1Dept. of Pediatrics, University of Minnesota, Minneapolis, MN, United States; 2University of Minnesota, Center for Magnetic Resonance Research, Minneapolis, MN, United States
In vivo 1H NMR NMR spectroscopy at 9.4T was used to investigate effects of morphine on the neurochemical profile of the developing rat hippocampus. Significant differences between pup exposed to the morphine (2 mg/kg/, twice a day, P3 P7) and their littermate controls were observed for multiple brain metabolites on postnatal day 8. These changes had resolved by P29. Biochemical changes indicated effects of morphine on inhibitory neurotransmission (GABA, Tau), glial development and myelination (Gln, myo-Ins), osmoregulation (myo-Ins, Tau) and antioxidant processes (GSH).These results indicate that morphine exposure during hippocampal development may lead to hippocampal-dependant cognitive deficits in premature infants.