John C. Nouls1, Manuel Fanarjian2, Bastiaan Driehuys1,3
1Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC, United States; 2Biomedical Engineering, Duke University, Durham, NC, United States; 3Radiology, Duke University Medical Center
We present a constant-volume small-animal ventilator that offers precise control of gas delivery, permits high-resolution hyperpolarized gas imaging, and captures the exhaled mixture containing 3He or 129Xe for recycling. The captured gas is compressed by a piston and stored in a cylinder to be sent for re-purification. The same ventilator can ventilate different small animals, simply by changing flow constrictors. The ventilator is inexpensive to duplicate and only uses off-the shelf components. By recapturing exhaled gas, it alleviates some of the costs associated with HP gas imaging.