Cicely Williams1, Dorit Granot2, Teodor Leahu1, Erin B. Lavik3, Erik M. Shapiro, 1,2
1Department of Biomedical Engineering, Yale University, New Haven, CT, United States; 2Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT, United States; 3Center for Translational Neuroscience, Case Western Reserve University, Cleveland, OH, United States
Critical to the use of magnetic particles for MRI-based cell tracking is that particles not interfere with cellular processes. This is especially the case with stem cells. In this work, we investigated the effect of magnetic cell labeling with various sized MPIOs on differentiation of mesenchymal stem cells and neural progenitor cells, down multiple cell lineages. Neural progenitor cells labeled with MPIOs differentiated into neurons and glia identically to unlabeled cells. Similarly, mesenchymal stem cells labeled with MPIOs were able to differentiate into adipocytes and osteocytes identically to unlabeled cells. Importantly, MPIOs remained intracellular during differentiation.