Tatjana Atanasijevic1, Jasmin Herz1, Dorian McGavern1, Alan P. Koretsky1
1NINDS, NIH, Bethesda, MD, United States
Viral infection of the central nervous system (CNS) is a common cause of inflammatory disease. Anti-viral T cells recruited from the periphery play a critical role in fighting CNS viral infections. The ability to track single T cells in the brain with MRI would help pre-clinical studies and open novel diagnostic possibilities. Here it is demonstrated that T cells can be labeled with MPIOs in short incubation times and sorted for cells that have internalized particles. These cells can be re-introduced into LCMV-infected mice and detected by MRI opening the possibility of tracking single T cells migrating in the brain.