Hirohiko Imai1,
Yuki Takayama1, Tetsuya Matsuda1
1Department
of Systems Science, Graduate School of Informatics, Kyoto University,
Sakyo-ku, Kyoto, Japan
We demonstrate a spatially encoded 2D heteronuclear multiple quantum coherence (HMQC) spectroscopy for imaging metabolites. A standard chemical shift imaging (CSI) incorporated with 2D 1H-13C HMQC preparation was implemented in a tumor-bearing mouse after sacrifice at 30min post injection of [U-13C]glucose. The 4D data acquisition (two spectral and two spatial dimensions) allows the identification of multiple metabolites with 1H sensitivity and 13C spectral resolution, and the visualization of their distribution throughout the whole body. As the result, the increase of relative amount of lactate was observed in the brain, tumor, and inferior limb.