In vivo quantitative cellular MR imaging guided by in vitro relaxation parameter measurements of magnetic labeled cells
Ulysse Gimenez 1 , Michle El Atifi 2 , Hlne Lajous 1 , Marie Bidart 2 , Pascal-Henry Fries 3 , Francois Berger 1 , and Hana Lahrech 1
CEA-Clinatec, Grenoble, France,
Institute of Neurosciences, La Tronche, France,
CEA, Grenoble, France
This study is focused on the cellular MRI using
ultra-small super-paramagnetic (USPIO) nanoparticles.
U937 macrophages are labeled with Molday Ion RhodamineB
(MIRB). Cell labeling was characterized in vitro, cell
proliferation and viability were not affected by the
MIRB internalization. In vitro experiments were
conducted at different cell concentrations and a
longitudinal in vivo study was achieved. This study
shows which MRI parameter (T2*, T2, or T1) is more
suitable to track magnetically labeled cells in vivo and
attests the ability to quantify labeled U937 cell
concentration in a longitudinal study.
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