STEAM-MiTiS: a new spectral editing method for the detection of scalar coupled metabolites and its application for the detection of Glutamate at 7T
Alessandra Toncelli 1,2 , Ralph Noeske 3 , Mauro Costagli 4 , Valentina Domenici 5 , Gianni Tiberi 4 , Mirco Cosottini 6 , and Michela Tosetti 7
Department of Physics, University of Pisa,
di Pisa, INFN, Pisa, Italy,
Science Laboratory, GE Healthcare, Berlin, Germany,
di Chimica e Chimica Industriale, University of Pisa,
di Ricerca Traslazionale e delle Nuove Tecnologie in
Medicina e Chirurgia, University of Pisa, Italy,
Maris Scientific Institute, Italy
We propose a novel spectroscopic method called STEAM-MIxing
TIme Subtraction (STEAM-MiTiS) which consists in
acquiring two different STEAM spectra with same TE, but
different TMs peculiar for each J-coupled metabolite.
This technique permits to cancel the contribution of
singlet lines and leaves the J-coupled signals, only.
The STEAM-MiTiS method has been optimized for the
detection of Glutamate. Simulation results yielded the
optimum parameter values that we used for both in-vitro
and in-vivo validation. Experiments indicated an almost
perfect cancellation of singlet and macromolecule
signals, and resulted in clear editing of the Glutamate
peak whose intensity scaled linearly with its
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