Restoring susceptibility induced MRI signal loss in rat deep brain structures at 9.4T and acquiring true whole brain scale fcMRI network
Rupeng Li 1 , Xiping Liu 2 , Jason W Sidabras1 1 , Eric S Paulson 3 , Andrzej Jesmanowicz 1 , and James S Hyde 1
Biophysics, Medical College of Wisconsin,
Milwaukee, WI, United States,
Medical College of Wisconsin, Milwaukee, WI, United
Oncology, Medical College of Wisconsin, Milwaukee, WI,
The aural cavity magnetic susceptibility artifact leads
to significant echo planar imaging, EPI, signal dropout
in rat deep brain that limits acquisition of functional
connectivity fcMRI data. We provide a method that
restores the EPI signal in deep brain. Needle puncture
introduction of a liquid-phase fluorocarbon into the
middle ear allows acquisition of rat fcMRI data without
signal dropout. We demonstrate that with seeds chosen
from previously unavailable areas, including the
amygdala and the insular cortex, we are able to acquire
whole brain scale networks, including the limbic system.
This tool allows EPI-based neuroscience and
pharmaceutical research in rat brain using fcMRI that
was previously not feasible.
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