Jae Mo Park1, Ralph E Hurd2, Shie-Chau Liu1, and Daniel M Spielman1
1Radiology, Stanford University, Stanford, CA, United States, 2Applied Sciences Laboratory, GE Healthcare, Menlo Park, CA, United States
Intracellular [lactate]:[pyruvate] is an important biomarker
of cytosolic redox-state, directly reflecting free cytosolic [NADH]:[NAD+].
Hyperpolarized [1-13C]alanine, which can be also transported across
the plasma membrane, is useful to measure relative concentrations of intracellular
pyruvate and lactate. In this work, we propose a simple method to assess
in vivo cytosolic redox-state using hyperpolarized [1-13C]alanine,
and demonstrate the ethanol-induced redox change in rat liver.