Abstract #3963

In Vitro Oxygen-17 NMR Spectroscopy of Cellular Metabolism at Ultra High Field

Ruomin Hu¹, Andreas Neubauer¹, Jorge Chacón-Caldera¹, Javier Uranga Solchaga¹, Christian Schuch², Tilo Gläser², Cordula Nies³, Eric Gottwald³, Stefan Giselbrecht⁴, and Lothar R. Schad¹

¹Computer Assisted Clinical Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany, ²NUKEM Isotopes Imaging GmbH, Alzenau, Germany, ³Institute for Biological Interfaces 5, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany, ⁴Department of Complex Tissue Regeneration, MERLIN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands

In this work we present a novel in vitro oxygen-17 NMR method using ¹⁷O-labeled glucose to investigate the metabolic process of cells cultivated in a MR-compatible microbioreactor. The metabolization of ¹⁷O-labeled glucose was demonstrated to produce MR-detectable H₂¹⁷O on the one hand and to not intervene with the inherent cellular physiology on the other hand, thus proving the method to serve as a neutral observation platform. The presented method has the potential to aid the modeling of fundamental physiological processes and to become a key element in cellular vitality assessment applications.

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