Abstract #4464

Using hyperpolarised 13C-MRS to explore murine skeletal muscle metabolism during exercise

M. Kate Curtis¹, Brianna J Stubbs¹, Vicky Ball¹, Lowri E Cochlin², Mark A Cole³, Jack J Miller^1,4, David P O'Neill¹, Kieran Clarke¹, Peter A Robbins¹, and Damian J Tyler^1,5

¹Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom, ²PulseTeq Ltd, Surrey, United Kingdom, ³Queen's Medical Centre, University of Nottingham Medical School, Nottingham, United Kingdom, ⁴Physics, University of Oxford, Oxford, United Kingdom, ⁵Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom

Previous techniques used to determine the hierarchy of skeletal muscle metabolic fuel selection have been unable to directly describe the changes in cellular metabolic flux during exercise. The aim of this project was to develop a technique that allows the simultaneous assessment of metabolism and function in exercising skeletal muscle in real-time, in an intact mouse, using hyperpolarized [1-¹³C] pyruvate MRS. Our results demonstrate that the technique is sensitive enough to distinguish differences in metabolic flux between the resting and exercising states shown by a significant increase in ¹³C label incorporation into bicarbonate during exercise.

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