Abstract #0167

[2-13C]dihydroxyacetone as a real-time, in vivo sensor of acute hepatic and renal metabolic response after a fructose and glucose challenge

Irene Marco-Rius¹, Cornelius Von Morze¹, Renuka Sriram¹, Peng Cao¹, Gene-Yuan Chang², Eugene Milshteyn¹, Robert A. Bok¹, Michael A. Ohliger¹, David Pearce², John Kurhanewicz¹, Peder E. Z. Larson¹, Dan B. Vigneron¹, and Matthew Merritt³

¹Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, ²Department of Medicine, Division of Nephrology, University of California San Francisco, San Francisco, CA, United States, ³Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States

Hyperpolarized [2-¹³C]dihydroxyacetone was used to investigate the hepatic and renal metabolic response to acute intravenous administration of glucose or fructose in rats in vivo. ¹³C-MR spectra were acquired before, 10 minutes and 80 minutes after the carbohydrate solution delivery. Changes in the metabolic products phosphoenolpyruvate (PEP) and glycerol 3-phosphate (G3P) were detected after fructose injection, while no metabolic perturbation was detected after the glucose injection. The observed effects possibly include ATP depletion and changes in the unlabelled pool sizes of glycolytic intermediates.

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