The feasibility of acquiring hyperpolarized 13C images from human hearts following injections of HP [1-13C]pyruvate solution has been recently demonstrated. Following the rapid multi-slice 13C imaging acquisition, 13C spectroscopic data were acquired from the whole heart to detect any residual hyperpolarized magnetization. 13C pyruvate, lactate, alanine and bicarbonate were observed in all 5 subjects. 13CO2 was also detected in two of the datasets. Good inter-subject consistency in the observed metabolite ratios from these spectra demonstrated the potential for changes in cardiac metabolism due to disease to be interrogated with simple whole-heart spectroscopy.