Optimizing parameters of electrical forepaw stimulation is necessary to investigate biophysical and molecular properties of fMRI signals. However stimulation parameters and anesthesia in mice are not well-defined. Here we introduced ketamine anesthesia in mice. Initially, forepaw stimulation frequency was refined with for CBV-weighted OIS experiments. To translate electrical stimulation parameters from OIS into BOLD fMRI, we tested two stimulation frequencies. Application of our sensory stimulus parameters evoked well-localized and robust BOLD fMRI signals in ketamine anesthetized mice.