Mice resting state functional connectivity (FC) studies are highly attractive given the large number of existing murine models of neuropsychiatric disorders. Currently, most of mouse rs-fMRI studies are carried out under anesthesia, to limit motion and animal distress. Yet, anesthetics affect brain FC. Currently, no reliable awake mouse brain FC reference is available. We compared mouse brain rs-fMRI patterns under medetomidine or isoflurane anesthesia and paralleled the experiments with static 18FDG-PET exams, where the tracer biodistributions occurred under isoflurane, medetomidine anesthesia or in conscious state, reflecting the brain glucose metabolism, as indirect measures of neuronal activity.