Diffusion weighted imaging showed the potential to evaluate thyroid disease. This study aimed to evaluate whether readout-segmented EPI (RS-EPI) can provide better image quality in imaging thyroid gland in comparison with single-shot EPI (SS-EPI), and to compare ADC values, acquired from RS-EPI with those of SS-EPI. Sixteen patients were examined using both techniques. There were significant differences in susceptibility, motion artifacts, except for detectability of thyroid nodules and ADC measurements between RS-EPI and SS-EPI. The present study found that the RS-EPI technique provides significant image quality improvement compared with SS-EPI in imaging thyroid gland at 3 Tesla.