QSM and DWI are sensitive to changes in MS lesions at various ages. We found chronic MS lesions had higher relative susceptibilities and lower relative ADC values as compared to new enhanced lesions. Combining QSM and ADC measurements could differentiate each two subtypes in four subtypes of lesions (nodular/shell enhanced lesions, rim+/- lesions). The pattern of QSM and ADC findings suggests that shell enhanced lesions have more demyelination than nodular enhanced lesions and rim- lesions. Combining QSM and ADC measurements might be a better way to differentiate MS lesions at various ages and provide more information of micro-changes of lesion.